Search results for "Locus Control Region"

showing 3 items of 3 documents

Evolution and genetic structure of the great tit (Parus major) complex

2003

The great tit complex is divided into four groups, each containing several subspecies. Even though the groups are known to differ markedly on morphological, vocal and behavioural characters, some hybridization occurs in the regions where they meet. The great tit has often been referred to as an example of a ring species, although this has later been questioned. Here, we have studied the genetic structure and phylogenetic relationships of the subspecies groups to clarify the evolutionary history of the complex using control region sequences of the mitochondrial DNA. The subspecies groups were found to be monophyletic and clearly distinct in mitochondrial haplotypes, and therefore must have h…

Mitochondrial DNAAsiaZoologySubspeciesBiologyDNA MitochondrialGeneral Biochemistry Genetics and Molecular BiologyEvolution MolecularSongbirdsMonophylyPhylogeneticsAnimalsCluster AnalysisPhylogenyDNA PrimersGeneral Environmental SciencemtDNA control regionParusGeographyGeneral Immunology and MicrobiologyPhylogenetic treeGeneral MedicineLocus Control Regionbiology.organism_classificationEuropeEvolutionary biologyHybridization GeneticGeneral Agricultural and Biological SciencesResearch ArticleRing speciesProceedings of the Royal Society of London. Series B: Biological Sciences
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Sequence polymorphism of mitochondrial DNA control region in Japanese.

1998

Sequence polymorphisms of the mitochondrial DNA (mtDNA) control region, hypervariable regions I and II, from 100 unrelated Japanese were determined by PCR amplification and direct sequencing. Sequences of 404 nucleotides for hypervariable region I and 379 nucleotides for region II were obtained. Variable sites (85 and 45) were revealed in region I and region II, respectively, as compared to the reference sequence, and a total of 96 different genetic patterns from both regions I and II were determined. A point mutation heteroplasmy was observed at the ratio of approximately 50:50 from one individual at the sequence position 151 showing a nucleotide transition from C to T. The probability of …

Mitochondrial DNAGenotypeSequence analysisPopulationMolecular Sequence DataBiologyDNA MitochondrialPolymerase Chain ReactionPathology and Forensic MedicineJapanHumansPoint MutationeducationDNA PrimersmtDNA control regionGeneticseducation.field_of_studyPolymorphism GeneticBase SequenceNucleic acid sequenceSequence Analysis DNALocus Control RegionHeteroplasmyHypervariable regionGenetics PopulationGenetic markerLawForensic science international
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Functional characterization of the sea urchin sns chromatin insulator in erythroid cells.

2005

Abstract Chromatin insulators are regulatory elements that determine domains of genetic functions. We have previously described the characterization of a 265 bp insulator element, termed sns, localized at the 3′ end of the early histone H2A gene of the sea urchin Paracentrotus lividus. This sequence contains three cis-acting elements (Box A, Box B, and Box C + T) all needed for the enhancer-blocking activity in both sea urchin and human cells. The goal of this study was to further characterize the sea urchin sns insulator in the erythroid environment. We employed colony assays in human (K562) and mouse (MEL) erythroid cell lines. We tested the capability of sns to interfere with the communi…

animal structuresGlobin enhancerChromatin insulator; Enhancer blocking; Erythroid transcription factor; Globin enhancerSp1 Transcription FactorSettore BIO/11 - Biologia MolecolareElectrophoretic Mobility Shift AssayDNA-binding proteinParacentrotus lividusCell LineMiceErythroid Cellshemic and lymphatic diseasesbiology.animalHistone H2AAnimalsHumansGATA1 Transcription FactorChromatin insulatorEnhancerMolecular BiologySea urchinTranscription factorbiologyGene Transfer TechniquesGATA1Cell BiologyHematologybiology.organism_classificationLocus Control RegionMolecular biologyChromatinChromatinCell biologyGlobinsEnhancer Elements GeneticSea UrchinsParacentrotusMolecular MedicineEnhancer blockingInsulator ElementsErythroid transcription factorOctamer Transcription Factor-1Blood cells, moleculesdiseases
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